Terminal Sialic Acids are an Important Determinant of Pulmonary Endothelial Barrier Integrity
In some experiments the bacterial surface was desialylated with 13· In the earlier set of experiments that utilized neuraminidase, we noted that, following neuraminidase treatment, there were typically fewer cells in the dish, indicating that cells had lost cell-cell and/or cell-matrix adhesions. Sialic acid quantitation was carried out using the Sialic Acid (NANA) Assay kit […]
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Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Cell. Biol. 4:1440-1448) ADH2 (Russell et al. “Conservatively modified variations” of a particular polynucleotide sequence refers to those polynucleotides that encode identical or essentially identical amino acid sequences, or where the polynucleotide does not encode an amino acid sequence, to essentially […]
US20110165626A1 – High Yield Production of Sialic Acid (Neu5ac) by Fermentation – Google Patents
To confirm correct sequences in plasmids constructed, the plasmids can be analyzed by standard techniques such as by restriction endonuclease digestion, and/or sequencing according to known methods. Yeast Integrating plasmids (e.g., YIp5) and Yeast Replicating plasmids (the YRp series plasmids) and pGPD-2. Media can be rich media, e.g., Luria broth or terrific broth, or synthetic […]
Similar to a Previous Study (19)
The data from these assays were complemented by a glycan array analysis which determined that AAV1 efficiently binds to NeuAcα2-3GalNAcβ1-4GlcNAc as well as glycoproteins containing NeuAcα2-3/α2-6Galβ1-4GlcNAc N-linked glycans. Sialic acids are typically found as terminal monosaccharides of glycans carried by cell surface glycoproteins. Mucin competition was carried out by pretreating rAAV with bovine submandibular gland […]
Similar to a Previous Study (19)
Whole cell lysate (20 μl) was combined with 80 μl of 0.05 N H2SO4 (hydrolysis reagent) and incubated at 80°C for 60 min. Samples were briefly centrifuged at 14,000 revolution/min (16,000 g), after which 20 μl of 1 M NaOH (neutralization reagent) was added and the mixture centrifuged again at 14,000 revolution/min. Afterwards, wells were […]
